| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Application | Recommended for use as a cell culture substratum. For a 24-well plate, use 230-250 μl/well. For a 96-well plate, use 50-100 μl/well. Thaw gel overnight at 2-8 °C before use. The thawed gel may be diluted up to two-fold with cold (2-8 °C) Dulbecco's Modified Eagle's Medium. Gel dilutions should be made before it is added to the plate. ECM will gel within 5 minutes at 20 °C. For prolonged manipulations, work should be conducted below 10 °C. Dispense gel to wells of a multiwell plate using pipettes pre-cooled to 2-8 °C. A gel forms at 37 °C and maintains this form with culture medium for at least 14 days. Cells may be plated on top of a thin gel layer (0.5 mm) or cultured inside a 1 mm layer. When cultured inside, cells should be added to the gel prior to plating at a recommended density of 3-4 × 104 cells per mL. To dissociate cells from the gel, use protease (dispase) dissolved in PBS without calcium, magnesium, and EDTA at a working concentration of 0.6-2.4 units/ml. |
| | Epithelial cells, endothelial cells, muscle cells, nerve cells, tumor cells |
| Caution | ECM gel may be stored up to 72 hours at 2-8 °C. |
| Other Notes | ECM gel is composed primarily of laminin, collagen type IV, heparan sulfate proteoglycan and entactin. Approximately 8-12 mg/ml basement membrane matrix protein in Dulbecco's modified Eagle's medium with 50 μg/ml gentamicin. |
